RESUMO
The free radical scavenging potential of roots of Thespesia lampas Dalz and Gibs was studied by different antioxidant models. Free radicals are implicated for more than 80 diseases including Diabetes mellitus, arthritis, cancer, ageing etc. In the treatment of theses diseases, antioxidant therapy has gained an utmost importance. Current research is directed towards finding naturally occurring antioxidant of plant origin. In Indian system of medicine, Thespesia lampas is an important medicinal plant and its root juice has been used in various ailments and as health tonic. To understand the mechanisms of pharmacological actions, the in vitro antioxidant activity of aqueous extract of Thespesia lampas was investigated for the activity of scavenging superoxide anion radicals, nitric oxide radical and lipid peroxidation assay. In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals, metal chelating, reducing power or inhibition of lipid peroxidation. These results clearly indicate that Thespesia lampas is effective against free radical mediated diseases
Assuntos
Raízes de Plantas , Antioxidantes/farmacologia , Plantas Medicinais , Superóxidos/química , Preparações de Plantas , Peroxidação de Lipídeos/efeitos dos fármacos , Óxido Nítrico/química , Ácido Ascórbico/análise , Indicadores e ReagentesRESUMO
In the present study, ethyl acetate, butanol and aqueous fractions derived from total methanol extract of Butea monosperma flowers were evaluated for radical scavenging activities using different in vitro models like reducing power assay, scavenging of 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, nitric oxide radical, superoxide anion radical, hydroxyl radical and inhibition of erythrocyte hemolysis using 2, 2' azo-bis (amidinopropane) dihydrochloride (AAPH). Methanol extract along with its ethyl acetate and butanol fractions showed potent free radical scavenging activity, whereas aqueous fraction was found to be devoid of any radical scavenging properties. The observed activity could be due to the higher phenolic content in the extracts (16.1, 25.29, and 17.74% w/w in methanol extract, ethyl acetate and butanol fractions respectively). HPTLC fingerprint profile of the ethyl acetate and butanol fractions were developed which would serve as reference standard for quality control of the extracts.
Assuntos
1-Butanol/química , Acetatos/química , Compostos de Bifenilo/química , Butea/química , Eritrócitos/efeitos dos fármacos , Flores/química , Sequestradores de Radicais Livres/isolamento & purificação , Radicais Livres/química , Hemólise/efeitos dos fármacos , Hidrazinas/química , Radical Hidroxila/química , Metanol/química , Óxido Nítrico/química , Oxirredução , Extratos Vegetais/farmacologia , Superóxidos/química , Água/químicaRESUMO
In vitro evaluation of antioxidant activities of Auricularia auricula showed significant inhibition of lipid peroxidation, and potent hydroxyl radical scavenging activity when compared with standard drug catechin. IC5o value of crude, boiled and ethanolic extracts of A. auricula represented 403, 510, and 373 microg/ml respectively in case of hydroxyl radical scavenging activity and 310, 572 and 398 microg/ml respectively in case of lipid peroxidation. Furthermore, crude, boiled and ethanolic extracts also increase significantly nitric oxide production (664, 191 and 850 pmole/mg dry wt/hr respectively) over the control. The present results revealed that A. auricula had potential therapeutic use.